Ruggedness is the degree of reproducibility of the results obtained under a variety of conditions. From stock solution, solutions containing 14 μg/ml of diazepam hydrochloride was prepared and analyzed PD-0332991 mouse by two different analysts using same operational and environmental conditions in different experimental periods. Percentage recoveries of the replicates were calculated. It is checked that the results are reproducible under differences in, analysts. The results are shown in Table 4. The method was found to be robust, although small deliberate changes in method conditions did have a negligible effect on the chromatographic behavior of the solute. The results indicate that changing
the detector wavelength had no large effect on the chromatographic behavior of diazepam hydrochloride. Even a small change of mobile phase composition (pH 3 ± 0.2), did not cause a notable change in the peak area of the used drug for this method. The results were presented in Tables 5 and 6. LOD and LOQ for diazepam were estimated by injecting a series of dilute solutions with known concentration. The parameters LOD and LOQ were determined on the basis of peak response and slope of the regression equation.
The LOD and LOQ of the drug were found to be 0.898 μg/ml and 2.72 μg/ml respectively. System suitability parameters can be defined as tests to ensure that the method can generate results of acceptable accuracy and precision. The requirements for system suitability are usually developed after method
development and validation has been completed. The system suitability Tryptophan synthase parameters like Theoretical plates (N), Resolution (R), Tailing factor (T) were calculated and compared GDC-0199 concentration with the standard values to ascertain whether the proposed RP-HPLC method for the estimation of diazepam in pharmaceutical formulations was validated or not. The results were shown in Table 7. A convenient, rapid, accurate, precise and economical RP-HPLC method has been developed for estimation of diazepam in bulk and tablet dosage form. The assay provides a linear response across a wide range of concentrations and it utilizes a mobile phase which can be easily prepared and diluent is economic, readily available. The proposed method can be used for the routine analysis of diazepam hydrochloride in bulk preparations of the drug and, in pharmaceutical dosage forms without interference of excipients. All authors have none to declare. “
“Since ancient times, plants and herbal preparations have been used as medicine. During the past few decades, traditional systems of medicine have become a topic of global importance. Current estimates suggest that, in many developing countries, a large proportion of the population relies heavily on traditional practitioners and medicinal plants to meet primary health care needs. Concurrently, many people in developed countries have begun to turn to alternative or complementary therapies, including medicinal herbs.1 Averrhoa bilimbi L.