4A). As with BC preparations, Bbil-TX (30 μg/ml) did not significantly change the twitch-tension responses of directly stimulated PND preparations pretreated with d-Tc (10 μg/ml) for 10 min before incubation with the toxin, when compared to control preparations (data not shown). Bbil-TX (30 μg/ml) caused slight depolarization of the resting membrane potential of mouse diaphragm muscle fibers after 120 min (control: −80 ± 1 mV vs. toxin: −66 ± 2 mV, n = 4 each; p < 0.05). In contrast, exposure of toxin-treated diaphragm muscle to carbachol (CCh; 12.5 μg/ml) resulted check details in membrane
depolarization from −66 ± 2 mV to −50 ± 3 mV (p < 0.05) after 15 min and a return to pre-CCh values (−67 ± 4 mV) after removal of CCh by washing. Bbil-TX (30 μg/ml) caused Selisistat a progressive decrease in the quantal content of EPPs from 94 ± 14 at t0 to 24 ± 3 at t60 (p < 0.05) ( Fig. 4B). In addition, there was a significant decrease in the MEPP frequency from 30 min onwards [from 26 ± 2.5 (basal) to 10 ± 1 after 60 min; n = 5; p < 0.05] ( Fig. 4C) with no alteration in the amplitude (0.9 ± 0.06 mV at t0 compared to 0.7 ± 0.06 mV at t60). Bbil-TX caused
limited myonecrosis in BC and PND preparations. Light microscopy showed that the level of damage correlated with the toxin concentrations used (1–10 μg/ml for BC and 3–30 μg/ml for PND). However, at none of these concentrations was the fiber damage as extensive as that caused by other Bothrops myotoxins. Fig. 5 shows the morphology of BC preparations incubated with Krebs solution (control, panel A) or the highest Bbil-TX concentration tested in this preparation (10 μg/ml, panel B) for 40 min and that of PND preparations incubated with Tyrode solution (control, panel C) or the highest Bbil-TX GBA3 concentration tested in this preparation (30 μg/ml, panel D) for 120 min. The changes in BC fiber morphology after 40 min of incubation with Bbil-TX included the presence of edematous (e) and/or hyperchromic (H) fibers and
loss of the normal cytoarchitecture that consisted of fiber bundles surrounded by a connective perimysial sheath (indicated by P in panel A) (panel B). Compared to control preparations (panel C), PND preparations incubated with the highest toxin concentration (30 μg/ml for 120 min) also showed edematous (e) and/or hyperchromic fibers, a loss of the muscle tissue cytoarchitecture, fibers with delta lesions (d) and condensed bands of myofibrils (asterisks; panel D). In agreement with the mild morphological alterations described above, Bbil-TX (10 μg/ml) caused a progressive release of CK from BC preparations (CK activity, IU/ml: 116 ± 17, 495 ± 55, 676 ± 87 and 710 ± 91 for 0 (basal), 15, 30 and 45 min post-toxin, respectively; n = 6; p < 0.05 for all intervals compared to basal values).