interrogans Icterohaemorrhagiae Icterohaemorrhagiae

LGL 471 human blood L. interrogans Canicola Canicola LGL Dorsomorphin 87 human urine L. kirschneri Grippotyphosa Grippotyphosa LGL 517 corpus vitreum, horse L. kirschneri Grippotyphosa Grippotyphosa LGL 518 corpus vitreum, horse L. kirschneri Grippotyphosa Grippotyphosa LGL 533 corpus vitreum, horse L. kirschneri Grippotyphosa Grippotyphosa LGL 539 corpus vitreum, horse L. kirschneri Grippotyphosa Grippotyphosa LGL 541 corpus vitreum, horse L. kirschneri Grippotyphosa Grippotyphosa LGL 112 human urine L. kirschneri Pomona Pomona LGL 511 corpus vitreum, horse L. kirschneri Pomona Pomona LGL 532 corpus vitreum, horse Spectra loaded into MALDI BioTyper™ 3.0 Version were

measured at the default settings. Unknown spectra were compared with the created reference library by using a score value, the common decadal logarithm for matching results. Results were analyzed following the score Doramapimod value system according to Bruker Daltonik GmbH (Bremen, Germany). Values from 3.00 to 2.30 indicate reliable species identification; values from 2.29 to 2.00 indicate reliable genus identification and probable species identification. Lower values stand for probable genus identification or no reliable match with the MSP database (http://​www.​bdal.​de). Statistical analysis using the ClinProTools software MALDI-TOF MS spectra were PLX-4720 datasheet exported into ClinProTools software version 2.2 (Bruker Daltonik GmbH, Bremen, Germany) to carry out statistical analysis. The software was used for visual comparison of the loaded spectra, as well as for identifying specific peaks of interest. First, 20 spectra for each of the investigated strains were loaded into the program and were automatically recalibrated. To compare individual strains, the same numbers of protein spectra were required to be analyzed using ClinProTools. Classification models SPTLC1 were automatically

generated. For this, the specific algorithms of the software, including QuickClassifier (QC)/Different Average, Supervised Neural Network (SNN) and the Genetic Algorithm were used. These algorithms proposed a list of discriminating peaks for the analyzed spectra according to the selected algorithm. Suggested peaks were visually evaluated and compared with the original spectra. This procedure was done for all algorithms and a manual report was created with the most relevant and reproducible mass peaks. Furthermore, statistical testing of the datasets was performed on the basis of principle component analysis (PCA) and results were displayed in a three-dimensional score plot, which was generated automatically by the software. Genotyping Strain confirmation was performed by sequencing all strains on the basis of a multi locus sequence typing as described by Ahmed et al. [33].