Quantitative RT-PCR validated the overexpression of several genes, including sFRP2, by the cancer-associated fibroblasts. Clinical data correlated stromal sFRP2 overexpression with poorer overall survival and chemoresistance in patients with high-grade late stage serous ovarian cancer, suggesting that sFRP2 promotes ovarian cancer progression. In vitro functional studies illustrate increased ovarian cancer cell find more line growth in response

to sFRP2. Our results illustrate a direct and specific signaling linkage from the tumor microenvironment to tumor cells that SRT1720 price contributes to tumor progression. Poster No. 114 Stromal Fibroblast-Derived Periostin Promotes Cancer Progression and Serves as Diagnostic and Poor Prognostic Factors in Cholangiocarcinoma Chanitra Thuwajit 1,7 , Kusumawadee Utispan 2,7, Yoshimitsu Abiko 3, Komkrid Jarngkaew4, Anucha Puapairoj 5,7, Siri Chau-in 6,7, Peti Thuwajit 1,7 1 Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-Noi, Bangkok, Thailand, 2 Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Muang, Khon Kaen, Thailand, 3 Department of Biochemistry and Molecular Biology,

Nihon University School of Dentistry at Matsudo, Matsudo, Japan, 4 Department of Pathology, Faculty of Medicine Siriraj Hospital, selleck Mahidol University, Bangkok-Noi, Bangkok, Thailand, 5 Department of Pathology, Faculty of Medicine, Khon Kaen University, Muang, Khon Kaen, Thailand, 6 Department of Surgery, Faculty of Medicine, Khon Kaen University, Muang, Khon Kaen, Thailand, 7 Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Muang, Khon Kaen, Thailand Cholangiocarcinoma (CCA) is a major health problem in Thailand. It is well recognized to contain abundant fibrous stroma with activated fibroblasts. Our group has recently isolated primary culture CCA fibroblast (Cf) from CCA tissues and revealed that Cf induced human biliary epithelial and CCA cell proliferation. However, molecular mechanism of fibroblasts in CCA remains unclear. Here, we indicated periostin (PN) secreted from cancer fibroblasts as diagnostic and prognostic factors, and had

carcinogenic role in CCA. By comparing gene expression profile of Cf and non-tumorigenic liver fibroblasts, 1,466 much genes were up-regulated whereas 495 genes were down-regulated in Cf. PN was verified up-regulated expression in Cf by real time PCR and western blotting. Immunohistochemistry of PN in CCA tissues (n = 139) revealed that PN was solely in tumor stromal fibroblasts. More than 80% of CCA cases had low to high level of PN, but slight expression was found in benign liver tissues and hepatocellular carcinoma. The overall survival of CCA patients with high PN expression was significantly lower than those who had low level (P = 0.029). Multivariate analysis indicated that high PN expression was an independent poor prognosis factor (P = 0.039).