The fourth gene, recA, codes for a product that initiates the formation of Holliday junction intermediates during homologous recombination [21]. Our ML and MP phylogenetic inferences based of these four gene sequences are in agreement with earlier findings by Kawamura et al. [2] and Poyart et al. [14] and corroborate the

S. thermophilus/S. vestibularis sister-relationship. Results Phylogenetic analyses of secA gene sequences We began our investigation of the branching order of the streptococci of the salivarius group by looking at phylogenetic trees Akt inhibitor inferred from the secA gene (Figure 1). As expected, the salivarius group comprising S. salivarius, S. thermophilus, and S. vestibularis was monophyletic in all the ML and MP bootstrap replicates. The S. thermophilus and S. vestibularis species monophylies were strongly supported by the ML and MP analyses, while support for the S. salivarius monophyly ranged from weak to moderate in the ML analyses and

strong in the MP analyses. Our phylogenetic analyses based on secA gene sequences strongly support the notion that S. vestibularis and S. thermophilus are closely related species. The node comprising these two species was retrieved in all the ML and MP bootstrap replicates, while the other two possible alternate topologies, CB-5083 cell line i.e., the S. salivarius/S. vestibularis and S. salivarius/S. thermophilus relationships, were not recovered

in any of the replicates. Figure 1 Branching order of members of the salivarius group as inferred from ML and MP analyses of secA gene sequences eltoprazine (2484 positions; 1261 variable, 1169 phylogenetically informative). The best ML tree computed with PHYML 3.0 under the GTR+Γ4+I model of nucleotide substitution is shown here. Bootstrap support for the major nodes is indicated over the corresponding nodes: ML values left, MP values right. PF-02341066 research buy Asterisks denote nodes that were retrieved in all the bootstrap replicates. Dashes indicate nodes that were retrieved in fewer than 50% of the bootstrap replicates. Streptococcal species belonging to the salivarius group are shown in orange (S. salivarius), blue (S. vestibularis), or green (S. thermophilus). Strains CCUG 7215 and CCUG 27306, which are categorized as Streptococcus vestibularis in the CCUG culture collection, are capable of using raffinose as the sole carbon source. This contradicts Whiley and Hardie’s [4] canonical S. vestibularis species definition. This metabolic trait is more a hallmark of the closely related Streptococcus salivarius species, to which the two strains belong. Other streptococcal species shown in black were outgroups. Branch lengths are drawn to scale.