Then the absorbance was measured at 515 nm. The capability to scavenge the DPPH radical was calculated using the following equation: DPPH scavenging activity(%)=Acontrol−AsampleAcontrol×100,where Acontrol

was the absorbance of the reaction in the presence of water and Asample the absorbance of the reaction in the presence of the extract. The extract concentration producing 50% inhibition (EC50) was calculated from the graph of the CHIR-99021 clinical trial DPPH scavenging effect against the extract concentration. Gallic acid, syringic acid and pyrogallol were used as standards. The 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS)) assay was done as previously described (Soares et al., 2009). Briefly, the stock solutions were 7.4 mmol/L ABTS + and 2.6 mmol/L potassium persulfate. The working solution was then prepared by mixing the two stock solutions in equal quantities and allowing them to react for 12 h at room temperature in the dark. this website The solution was then diluted by mixing 1 mL ABTS + solution with 60 mL methanol to obtain an absorbance of 1.1 at 734 nm. A fresh ABTS + solution was prepared for each assay. A volume of 150 μL of each extract (final concentrations from 5 to 100 μg/mL) was allowed to react with 2850 μL of the ABTS + solution (final concentration of 0.02 mmol/L) for 2 h in the dark.

Finally, the absorbance at 734 nm was measured. Distilled water was used instead of mushroom extracts as a control. The capability to scavenge the ABTS radical was calculated using PRKD3 the following equation: ABTS scavenging activity(%)=Acontrol−AsampleAcontrol×100,where Acontrol

was the absorbance of the reaction in the presence of water and Asample the absorbance of the reaction in the presence of the extract. The extract concentration producing 50% inhibition (EC50) was calculated from the graph of the ABTS scavenging effect against the extract concentration. Gallic acid, syringic acid and pyrogallol were used as standards. The ferrous ion chelating ability of extracts was determined as described previously described (Soares et al., 2009). Briefly, a sample (0.7 mL) of each extract was diluted in 0.7 mL of distilled water and mixed with 0.175 mL of FeCl2 (0.5 mmol/L) and the absorbance (A0) was measured at 550 nm. After, the reaction was initiated by the addition of 0.175 mL ferrozine (0.5 mmol/L). The mixture was shaken vigorously for 1 min and left standing at room temperature for 20 min when the absorbance (A1) was again measured at 550 nm. The percentage of inhibition of the ferrozine–Fe2+ complex formation was calculated as follows: chelating ability(%)=A0−A1A0×100. A lower absorbance indicates higher chelating ability. The extract concentration producing 50% chelating ability (EC50) was calculated from the graph of antioxidant activity percentage against the extract concentration. Gallic acid, syringic acid and pyrogallol were used as standards.