1, 3, 6, 19 and 21, who were infected by 2 genotypes, still have a major one across both gastric niches, and that was also true in 2 (no. 14, 27) patients having 3, and 1 (no. 17) patient having 4 genotypes represented in their infections. – indicating that the patients have non-dominant babA and babB Selleckchem Baf-A1 genotype in the isolates of antrum or corpus. The patients’ number was according to our previous study [22]. Among those 12 patients infected with more than one genotype (Table 2), VX-680 in vitro the frequency of the major dominant genotype, A B combined with AB AB, in the antrum
was higher compared with that in the corpus (75% [9/12] vs. 16.2% [2/12], p = 0.012, odds ratio: 15). However, 6 of 12 patients lacked a dominant genotype in their corpus isolates. Sequence analysis and comparison At locus A, each patient’s antrum and corpus isolates had specific substitutions
SBE-��-CD order of amino acids in the region of BabA (Figure 2 and Table 3). However, there was no obvious difference between the antrum and corpus isolates in the sequencing region, except from patient no. 27 (amino acid 134 and 198). We also found 5 different nonsynonymous substitutions at amino acid 161 in 6 patients’ isolates, as compared with strain J99. The same scenario (sequence specificity in individual patients’ strains but not between the antrum and corpus isolates) was in the babB sequences. Figure 1 PCR banding patterns of babAB genotypes. (A) Primer pairs used for gene detection at locus A and B. The forward primers, HypDF1 and S18F1, located in the upstream region of babA or babB, are paired with BabAR1 or
BabBR1 medroxyprogesterone primers to determine whether the gene at locus A and B is babA or babB. (B) PCR banding patterns of genotype A B, AB B, A AB and AB AB. The AB B genotype showed two bacterial populations in the single-colony isolate, the dominant as babA and the minor as babB, at locus A. The strain with A AB genotype represented a dominant population of babB and a minor population of babA at locus B. The combination of AB B and A AB was defined as an AB AB genotype. Lane M1, a 100 bp molecular marker; lane M2, l HindIII marker. The size of PCR products at locus A and B was 2.1-2.6 kb and 1.0-1.5 kb, respectively. Table 3 The amino acid substitutions in BabA encoded by babA at locus A The location of amino acid substitutions Case No.