CagA is considered to be an important bacterial virulence factor associated with both gastric adenocarcinoma and duodenal ulcer disease [2, 5, 11, 12, 26]. The number and pattern of phosphorylation motifs seem to further stratify the risk associated with individual strains [18, 27]. It

has been demonstrated that H. pylori CagA EPIYA patterns have a significant geographic variability and closely follow patterns of historical human migrations. EPIYA D is a characteristic Asian EPIYA pattern that virtually does not occur in the Western H. pylori strains [28]. The Brazilians form an unique Western population because, despite the multiple origins and the consequent wide diversity of phenotypic appearance, there has been a substantial degree of inter-ethnic breeding and

thus most individuals cannot be ascribed to any of the founding groups on the basis of genetic background, rather they carry about 33% of genes from each of the major NVP-LDE225 nmr races that historically composed the country (Caucasians, Africans and Amerindians) [29]. With this background, it would be expected to find some CagA EPIYA D in our H. pylori strains, as it has been detected among Amerindians (in keeping with the theory that initially people from Asia populated the Americas migrating from the East Asia), but we did not detect any EPIYA D in our population. Unfortunately, there are few studies in respect to the association between EPIYA C number and H. pylori associated diseases in Western populations with discordant results among them. Basso et al. [19] showed that higher number of EPIYA C segments was associated with gastric carcinoma in a Caucasian population Akt inhibitor C1GALT1 from Italy, similarly to the results of Yamaoka et al. [18] evaluating American patients from Texas. Otherwise, no association was observed when Colombian patients were evaluated in the Yamaoka’s study [18] in accordance with the results obtained by Acosta et al. [22], whereas Sicinschi et al. [21] observed associations between increased EPIYA C segments and precancerous lesions. Also, non-conclusive results published by

Argent et al. [20] evaluating 44 strains from African patients the authors showed tendency of association between CagA with two or more EPIYA C segments and gastric cancer. These differences may be explained by different study designs, sample size, populations and geographical diversity of H. pylori markers of pathogenicity, in respect to the CagA EPIYA pattern, highlighting the need of studying different geographical regions. The results of this study showed that higher number of EPIYA C segments is associated with gastric cancer and with pre-malignant lesions, atrophy and intestinal metaplasia of the corpus mucosa in the group of patients with gastritis. In agreement with these findings, we also demonstrated that serum concentration of PGI was twice decreased in the patients infected by cagA-positive strains with two or three EPIYA C motifs.