The data obtained were compared with available sequences in the GenBank database (National Institute of Health). Point mutations in ALB1, encoding a pentaketide synthase which is involved in the early steps of this metabolic pathway, were identified for pigmentless isolates IHEM 2508 and 9860 (Table 3). More precisely, a nonsense mutation was identified for isolate IHEM 2508, which caused truncation of the enzyme by173 amino acid residues at its C-terminus, leading to the loss of the thioesterase/claisen cyclase (TE/CLC) domain in particular. A deletion was detected for IHEM 9860, leading to a click here shift in the Selleck CHIR 99021 reading frame from the amino

acid at position 1678, and thus to the loss of an acyl carrier protein (ACP) domain and the TE/CLC domain. The metabolic pathway was blocked at a later {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| step for the brownish isolate IHEM 15998. Sequencing of the different genes showed an insertion in the ARP2 gene, which encodes a hydroxynaphthalene reductase (Table 3). This mutation led to a shift in the reading frame after the amino acid at position 140, and consequently

to the loss of the dehydrogenase/reductase domain. The missense mutation (C1391G) found in ABR2 for IHEM 9860 led to the replacement of a glutamine (Gln) by a glutamic acid (Glu) at position 217. The effect of this mutation on the protein function is not clear. Table 3 Mutations detected in the genes involved in melanin biosynthesis for A. fumigatus isolates IHEM 2508, 9860 and 15998 Isolate Point mutations in genesa   ALB1 AYG1 ARP2 ARP1 ABR1 ABR2 IHEM 2508 (FJ406465) Selleck HA-1077 (FJ406471) (FJ406477) (FJ406483) (FJ406489) (FJ167495)   G1203Ab C1017Ab G843T – A677Cb A582Gb   A4636Tb   T1053Cb         T5639Cb             C6739T           IHEM 9860 (FJ406466) (FJ406472) (FJ406478) (FJ406484) (FJ406490) (FJ167496)   C720T C1017Ab T1053Cb – A677Cb A582Gb   G1203Ab       T594A     A4636Tb       C1391G     T5639Cb             G5854X             G5904A           IHEM 15998 (FJ406468) (FJ406474)

(FJ406480) (FJ406486) (FJ406492) (FJ167498)   G1203Ab C1017Ab X751G – A677Cb A582Gb   A4636Tb   G843T         T5639Cb   T1053Cb       a Mutations are described as follow: first letter corresponds to the nucleotide present in the GenBank database sequence for the corresponding gene (accession numbers; AF025541, AF116902, AF099736, AFU95042, AF116901, AF104823 for ALB1, AYG1,ARP2, ARP1, ABR1 and ABR2, respectively), the number represents the relative position from the start of the reference sequence, and the second letter represents the nucleotide found in the gene sequence for isolates IHEM 2508, 9860 or 15998. The letter X placed after the number indicates a deletion of the corresponding nucleotide, and the same letter placed before the number corresponds to an insertion. The missense mutations found in the different gene sequences are underlined. Nonsense mutations, insertions and deletions are in bold type.