The purified recombinant polypeptide was employed to develop an ELISA test for the detection of TGEV-like CCoV-specific antibodies in dog sera. Four canine sera positive for TGEV-like CCoV, six sera positive to classical CCoV-II strains and 10 negative control sera were examined. The recombinant S’ was not recognized by antibodies to classical CCoV-II, as only sera from dogs infected experimentally with TGEV-like CCoV reacted strongly with the recombinant S’ polypeptide whereas dog sera with antibodies
to classical CCoV-II CUDC-907 nmr did not react. As classical CCoV-II and TEGV-like CCoVs are related antigenically, the recombinant S’ ELISA is a useful method to investigate serologically the prevalence of TGEV-like CCoVs in dogs. (C) 2009 Elsevier B.V. All rights reserved”
“Introduction: Molecular imaging of human epidermal growth factor receptor type 2 (HER2)-expression in malignant tumors provides potentially important information for patient management. Affibody molecules have shown to be suitable tracers for imaging applications using single photon emission computed tomography or positron emission tomography. Results from an earlier evaluation of the application of site-specific Tc-99m-labeling of the Affibody molecule, Z(HER2:2395)-C, were favorable.
Methods: As a preparation for clinical application of this tracer, we have developed and evaluated a robust single-vial freeze-dried kit, allowing labeling of the Affibody molecule, Z(HER2:2395)-C, with Tc-99m.
Results:
The composition of the kit [containing glucoheptonate, EDTA and tin(11)-chloride], as well as the protein amount and the pertechnetate volume were optimized for a high labeling yield (>90%) and minimal presence DNA Damage inhibitor of reduced hydrolyzed
technetium colloids (<1%). The specificity to HER2 receptors, the binding competence and the stability in phosphate-buffered saline and murine serum were verified in vitro. The shelf-life was also evaluated in vitro, showing no reduction in labeling yield or binding capacity to HER2-expressing cells after over 400 days of storage of the single-vial freeze-dried kit.
Conclusions: Z(HER2:2395)-C labeled with Tc-99m using the lyophilized kit was stable and resulted in a favorable biodistribution in an in vivo evaluation in normal Naval Medical Research Institute mice. (C) 2010 Elsevier Inc. All rights reserved.”
“A PCR assay that covers animal and human influenza Doxacurium chloride A, B and C viruses, i.e., most of Orthomyxoviridae, is needed. Influenza types are distinguished based on differences in the nucleoprotein (NP) present in the virus. Conserved NP regions were therefore used to design a TaqMan (R)-based triplex reverse transcription real-time PCR method. Variability of influenza A within the probe target region mandated the development of a novel molecular beacon, the “”Mega”" molecular beacon (MegaBeacon: MegB), for the detection of influenza A with this method. MegaBeacon is a mismatch-tolerant molecular beacon that is also a TaqMan (R) probe.