Through immunohistochemistry we detect the expression of FAK and phosphorylated
FAK (p-FAK) protein in tumor tissue and make double blind IHC score. By method of microRNA extraction and Real-time PCR we detect expression of miRNA-7 in tumor tissue and adjacent tumor tissue. Through the patient clinical data combined with the characteristic of expression of FAK , p-FAK protein and miRNA-7,we compare differential levels of expression of each index and analyse the correlation of different indexes with patient clinical data in tumor and adjacent groups by the statistical JQ1 mw software SPSS 18. Results: 1. The expression of FAK and p-FAK (Tyr397) is significantly higher than that of the adjacent tissue. Expression of FAK and p-FAK with lymph node metastasis in colorectal cancer was significantly higher than that
of without lymph node metastasis.2. The expression of miRNA-7 in colorectal cancer tissues is significantly lower than the adjacent tissue miRNA-7 expression, and there was significant difference between the two groups by statistical analysis.3. miRNA-7 expression of cancer tissues of patients with TNM stage III/IV is significantly lower than that INCB018424 order of patients with stage I / II, and expression of miRNA-7 with lymph node metastasis in colorectal cancer is significantly lower than that of without lymph node metastasis. Expression level of FAK is negatively correlated with the expression of miRNA-7 level in colorectal cancer. Conclusion: Expression
of FAK and p-FAK is related with tumor growth and lymph node metastasis. High expression level of FAK i negatively correlated with the expression of miRNA-7 level in colorectal cancer, and miRNA-7 is closely related to colon cancer progression, metastasis by combining with the patient clinical date. In summary, the lower miRNA-7 expression level, the later colorectal cancer progression of patients, the poorer the prognosis. Key Word(s): 1. Colorectal cancer; 2. microRNA-7; 3. FAK; 4. Lymph metastasis; Presenting Author: XIAOHUA LI Additional Authors: YING ZHANG, YONGQUAN SHI, YONGZHAN NIE, QINGCHUAN ZHAO, JIE DING, KAICHUN WU, DAIMING FAN Corresponding Author: XIAOHUA MCE LI Affiliations: Xijing Hospital of Digestive Diseases; Xijing Hospital of Digestive Diseases, the Fourth Military Medical University; Xijing Hospital of Digestive Diseases, the Fourth Military Medical University,; Xijing Hospital of Digestive Diseases, the Fourth Military Medical University,; Xijing Hospital of Digestive Diseases, the Fourth Military Medical University,; Xijing Hospital of Digestive Diseases, the Fourth Military Medical University, Objective: Peritoneal dissemination is the overwhelming cause of mortality in patients with gastric cancer. The absence of specific markers for peritoneal metastasis in gastric cancer has made the successful surgical treatment of human gastric cancer difficult.