We chose this race because it was the largest 24-hour running race in the Czech Republic with the highest number of participants and we also wanted to compare ultra-runners with ultra-MTBers. The lap was 1 km, situated around an athletic stadium on asphalt with 1 m rise. The athletes could consume food and beverages ad libitum from a buffet provided by the organizer with warm and cool food like apples, ananas, oranges, dried fruit, potatoes, rice, cookies, bread, pasta, porridge, soup, water, tea, isotonic drinks, fruit juices, cola, broth, and coffee. Runners could place their own camping tables
and chairs with personal belongings, food and drinks in a Protein Tyrosine Kinase inhibitor designated area. The maximum LY3023414 manufacturer temperature was +18°C, the minimum temperature was +10°C, and the average temperature was +12 (3)°C. On average 15 (5) mm
of precipitation was recorded and relative humidity changed from 58 till 94% over the duration of the race. The ,Trilogy Mountain Bike Stage Race‘ (R4), the first MTB stage race held in the Czech Republic, took place from July 4th 2012 till July 8th 2012 in Teplice nad Metují. This four day race consisted of a prologue and three stages, each of which had a completely different character. We chose this race Selleck BMN673 to compare 24-hour races with a stage race. The difficulty of this race was similar to other stage MTB races in Europe. The prologue covered 3 km with 300 m difference in elevation, Stage 1 covered 66 km with 2,200 m of altitude to climb, Stage 2 was 63 km in length with 2,300 m difference in elevation Interleukin-2 receptor and Stage 3 was 78.8 km with 3,593 m. Stage routes were characterized by a large number of individual trails which only interrupted
by a necessary minimum of road sections. Aid stations located along the routes offered beverages such as hypotonic sports drinks, tea, soup, caffenaited drinks, water, fruit, vegetables, energy bars, bread, soup, sausages, cheese, bread, chocolate and biscuits. During the prologue the average temperature was +32 (1)°C and relative humidity was 55 (2)% over the duration of the race. At Stage 1 the maximum temperature was +33°C, the minimum +22°C, the average temperature was +27 (7)°C and relative humidity changed from 80% at the start till 48% at the end of the race. At Stage 2 the maximum temperature was +30°C, the minimum +22°C, the average temperature +25 (3)°C and relative humidity changed from 83% till 60% over the duration of the race. At Stage 3 the maximum temperature was +31°C, the minimum +19°C, the average temperature was +25 (2)°C and relative humidity changed from 85% till 37% over the duration of the race. Procedures and calculations The procedures of pre- and post-race measurements were identical. At first, pre-race anthropometry of the subjects was assessed. Athletes were measured after voiding their urinary bladder.